Study on the Protective Effect of Hydrogen-rich Water on Acute Liver Injury Model in Mice
Objective To study the protective effect of hydrogen-rich water on acute liver injury in mice.Methods Sixty male KM mice were randomly divided into blank group,model group,positive control group,hydrogen-rich water low dose group,hydrogen-rich water dose group and hydrogen-rich water high dose group.Except for the blank group,mice in each group were intraperitoneally injected with 0.3%CCl4 to prepare acute liver injury model.The morphological changes of the liver were observed,the liver index was calculated,and the pathological changes of the liver were observed and scored by HE staining.The concentrations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL-F)and direct bilirubin(DBIL-F)in serum of mice were detected.In addition,the liver tissues(n=5)of mice in the blank control group,model group and hydrogen-rich water dose treatment group were collected for transcriptome sequencing.Results Compared with the blank group,the color of liver tissue in the model group became lighter,the surface appeared texture,and the pathological score increased.Compared with the model group,the liver tissue injury score of the hydrogen-rich water group decreased,the liver index decreased,and the concentrations of ALT,AST,TBIL-F,and DBIL-F in the serum decreased.In the hydrogen-rich water group,100 genes were up-regulated and 194 genes were down-regulated,a total of 294 differentially expressed genes.Conclusion Hydrogen-rich water can reduce the concentration of ALT,AST,TBIL-F and DBIL-F in serum,make the gene expression characteristics in liver tissue close to the blank control group,reduce the degree of liver injury,and finally play a protective role in the mouse model of acute liver injury.
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