青藤碱调控LncRNA-HEIH表达抑制人食管癌细胞迁移和侵袭
Sinomenine Inhibits Migration and Invasion of Human Esophageal Cancer Cells by Regulating LncRNA-HEIH Expression
陈中凯 1伍贤权 1敬家辉 1龙春晖 1李龙 1陈伟毅1
作者信息
- 1. 湖南医药学院医学院,湖南 怀化 418000
- 折叠
摘要
目的 探讨青藤碱(SIN)对食管癌细胞迁移、侵袭的影响及对LncRNA-HEIH的调控作用.方法 采用不同浓度青藤碱处理食管癌TE-1 细胞,si-NC、si-HEIH 分别转染TE-1 细胞,pcDNA、pcDNA-HEIH 分别转染TE-1 细胞后加入青藤碱处理细胞;CCK8 检测细胞活力,划痕实验检测细胞迁移率,Transwell实验检测侵袭细胞数,qRT-PCR检测HEIH表达量.结果 青藤碱 25、50、100 mg/L对TE-1 细胞活力无明显影响(P＞0.05),在青藤碱 25、50、100 mg/L的作用下,TE-1 细胞迁移率和侵袭细胞数显著降低,差异有统计学意义(P＜0.01),在青藤碱 25、50、100 mg/L的作用下,TE-1 细胞HEIH 表达显著降低,差异有统计学意义(P＜0.01).转染si-HEIH可显著降低TE-1 细胞迁移率和侵袭细胞数,差异有统计学意义(P＜0.01),过表达HEIH可显著逆转青藤碱降低TE-1 细胞迁移率和侵袭细胞数的作用(P＜0.01).结论 青藤碱通过抑制HEIH表达,抑制食管癌细胞迁移和侵袭.
Abstract
Objective To investigate the effect of sinomenine(SIN)on the migration and invasion of esophageal cancer cells and the regulation of LncRNA-HEIH.Methods Esophageal cancer TE-1 cells were treated with different concentrations of sinomenine.TE-1 cells were transfected with si-NC and si-HEIH,respectively.TE-1 cells were transfected with pcDNA and pcDNA-HEIH,respectively,and then treated with sinomenine.CCK8 was used to detect cell viability,scratch test was used to detect cell migration rate,Transwell test was used to detect the number of invasive cells,and qRT-PCR was used to detect HEIH expression.Results Sinomenine 25,50 and 100 mg/L had no significant effect on the viability of TE-1 cells(P>0.05).Under the action of sinomenine 25,50 and 100 mg/L,the migration rate and invasive cell number of TE-1 cells decreased significantly(P<0.01).Under the action of sinomenine 25,50 and 100 mg/L,the expression of HEIH in TE-1 cells decreased significantly(P<0.01).Transfection of si-HEIH significantly reduced the migration rate and the number of invasive cells of TE-1 cells(P<0.01).Overexpression of HEIH significantly reversed the effect of sinomenine on reducing the migration rate and the number of invasive cells of TE-1 cells(P<0.01).Conclusion Sinomenine inhibits the migration and invasion of esophageal cancer cells by inhibiting the expression of HEIH.
关键词
青藤碱/HEIH/迁移/侵袭/食管癌Key words
Sinomenine/HEIH/Migration/Invasion/Esophageal cancer引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据