Effects of Cadmium Exposure on Testiculus of Mice Offspring at Different Weeks of Age
Objective To study the effects of cadmium exposure before pregnancy,during pregnancy and lactation on testiculus development of male offspring and its potential mechanism.Methods Ten-week-old C57BL/6J male and female mice were randomly divided into control group and cadmium exposure group.At the age of 12 weeks,the two groups of mice were mated according to the ratio of female/male mice of 2∶1.The pregnant rats in the cadmium exposure group continued to be fed with low-dose cadmium until the end of lactation.The male offspring were fed to weaning at 3 weeks of age.After weaning,some 3-week-old male pups terminated the experiment,and the other part continued to be fed to 10 weeks of age according to the parental grouping method.Results Compared with the control group,the cadmium concentration in the testiculus tissue of the 3-week-old and 10-week-old mice in the cadmium exposure group was significantly increased,and that in the 10-week-old mice were significantly higher than that in the 3-week-old mice(P<0.05).There was no significant damage to the testiculus tissue of the 3-week-old mice in the cadmium exposure group,and there was no significant change in the expression ratio of cleaved caspase-3/caspase-3(P>0.05).In the cadmium exposure group,the spermatogenic epithelium of the testis of the 10-week-old mice was disordered,the spermatogenic cells were detached,and the expression ratios of cleaved caspase-3/caspase-3 and BAX/BCL-2 were significantly increased(P<0.05).Conclusion Cadmium can enter the male mice offspring through the placental barrier and accumulate in the testiculus,and has no obvious damage to the development of the testiculus in the juvenile stage,but its continuous accumulation can cause testiculus damage in adolescent male mice by increasing testiculus cell apoptosis.
国家科技期刊平台
NSTL
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