Value of Enzyme-linked Immunosorbent Assay and RT-PCR in the Detection of Hepatitis B Virus in Blood Samples
Objective To analyze the value of enzyme-linked immunosorbent assay and RT-PCR in the detection of hepatitis B virus in blood samples.Methods A total of 63 patients with suspected hepatitis B diagnosed and treated in our hospital from March 2020 to March 2023 were selected as the research objects.All patients were detected by enzyme-linked immunosorbent assay(ELISA)and fluorescence quantitative PCR(RT-PCR).The window period,diagnostic efficacy(sensitivity,specificity,accuracy,positive predictive value,negative predictive value),HBV-DNA results detected by PCR(large three positive,small three positive,acute or chronic infection period),HBV-DNA and serum markers(HBsAg,HBeAg)positive rate were observed.Results The window period of RT-PCR was(21.12±4.54)d,which was shorter than(36.20±5.84)d of ELISA(P<0.05).The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of RT-PCR were higher than those of ELISA(P<0.05).The positive rate of HBV-DNA and HBV-DNA quantification in patients with large three positive were higher than those in patients with small three positive,acute or chronic infection,and patients with small three positive were higher than those in patients with acute or chronic infection(P<0.05).BV-DNA positive patients were mostly HBsAg carriers,but there was no significant difference in the coincidence rate of HBsAg and HBeAg detection(P>0.05).Conclusion ELISA method and RT-PCR method have certain value in the detection of hepatitis B virus in blood samples.RT-PCR method has a small window period,which can improve the diagnostic efficiency and quantitatively detect HBV-M.However,the ELISA method can be used to qualitatively detect HBV-M.The two have mutual effects and can be combined in clinical practice to improve the reliable reference for the treatment of hepatitis B.
Enzyme-linked immunosorbent assay methodRT-PCR methodBlood samplesHepatitis B virus
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