摘要
目的 利用生物信息学筛选和分析小儿溃疡性结肠炎(ulcerative colitis,UC)的潜在基因生物标志物.方法 从基因表达数据库GEO中下载炎症性肠病数据集GSE126124 的表达谱数据.使用GEO2R获取基因数据集中小儿UC组织与相应正常组织的差异表达基因(differentially expressed genes,DEGs).DAVID、STRING数据库对DEGs进行生物学功能、通路富集分析和蛋白质-蛋白质相互作用分析.Cytoscape基于蛋白质-蛋白质相互作用网络鉴定关键基因,KEGG分析关键基因的通路富集情况.结果 获得了 153 个DEGs,包括 92 个高表达基因和 61 个低表达基因.高表达DEGs显著富集的生物学功能和通路有外部刺激反应、金黄色葡萄球菌感染和IL-17 信号通路等.低表达的DEGs显著富集的生物学功能和通路有转运、膜的成分和代谢通路等.此外,10 个DEGs可作为关键基因,包括Cxcl1、Cxcl2、Cxcl10、Cxcr2、Il1rn、Fcgr3a、Cxcr1、S100a12、Ido1 和Ccl24.关键基因显著富集的通路有趋化因子、病毒蛋白与细胞因子及受体的作用、幽门螺杆菌感染上皮细胞、IL-17 和TNF信号通路.结论 本研究发现了小儿UC的153个DEGs,其中10个关键基因有可能在小儿UC的发生发展中起重要作用.
Abstract
Objective Bioinformatics analysis was performed to screen and identify the underlying gene biomarkers in pediatric ulcerative colitis(UC)patients.Methods GSE126124 dataset,the mRNA expression profile of inflammatory bowel disease,was downloaded from the gene expression omnibus(GEO)database.GEO2R was utilized to obtain differentially expressed genes(DEGs)between pediatric ulcerative colitis tissues and corresponding normal tissues in the dataset.Functional and pathway enrichment analysis and protein-protein interaction analysis of DEGs were conducted using the DAVID and STRING database.The Cytoscape software was used to analyze protein-protein interaction network and hub genes.At last,the KEGG analyzed the biology and pathway enrichment of hub genes.Results A total of 153 DEGs were obtained,including 92 up-regulated and 61 down-regulated genes.Functional and pathway enrichment analysis showed that up-regulated DEGs were significantly enriched in the external stimulus,staphylococcus aureus infection and IL-17 signaling pathway.Functional and pathway enrichment analysis showed that down-regulated DEGs were significantly enriched in the transport,membrane composition and metabolic pathway.Furthermore,10 DEGs were considered hub genes,including Cxcl1,Cxcl2,Cxcl10,Cxcr2,Il1rn,Fcgr3a,Cxcr1,S100a12,Ido1 and Ccl24.Pathway enrichment analysis showed that hub genes were significantly enriched in the chemokines,the interaction between viral proteins and cytokines and receptors,epithelial cells infected by Helicobacter pylori,IL-17 and TNF.Conclusion This research found 153 DEGs,in which 10 hub genes may play an important role in the occurrence and development of pediatric UC.
基金项目
桂林市技术应用与推广计划项目(202012E15593)
国家科技期刊平台
NSTL
维普
万方数据