Anti-CCL20 monoclonal antibody suppresses the overexpression of pro-inflammatory cytokines in sarcoido-sis accompanied by serum amyloid A
Objective This study aims to investigate whether the anti-CCL20 monoclonal antibody inhibits the expression of pro-inflammatory cytokines in peripheral blood mononuclear cells(PBMCs)with high levels of serum amyloid A(SAA)associated with sarcoidosis.Methods PBMCs were extracted from both healthy individuals and patients with sarcoidosis.In addition to the blank control group and the sarcoidosis PBMC controlgroup,the PB-MCs from the sarcoidosis group were separately treated with SAA,SAA + anti-CCL20 monoclonal antibody,SAA + NF-κB inhibitor BAY11-7082,or dexamethasone.After cell culture for 48 hours,cells from each group were collected.The expression levels of cyto-kines(IL-17A,IL-23,IL-6,CCL20,and TGF-β1)in the cell su-pernatant were determined using ELISA.The mRNA expression levels of CCR6,IL-17,ROR-γt,and Foxp3 in the cells were assessed using RT-PCR.The protein expression levels of p-NF-κB and NF-κB in the cells were examined by Western blot.Results In the PBMC+SAA+anti-CCL20 monoclonal antibody group,the levels of IL-17A,IL-23,and IL-6 in the culture supernatant were significantly decreased compared to that in the PBMC+SAA group(P＜0.01),while TGF-β1 content was significantly increased(P＜0.01).In comparison to the PBMC+SAA group,the PBMC+SAA+anti-CCL20 monoclonal antibody group exhibited a significant reduction in the mRNA levels of CCR6,IL-17A,and ROR-γt(P＜0.01),with no significant difference of Foxp3 mRNA(P＞0.05).The expression level of p-NF-κB protein in the PBMC+SAA+anti-CCL20 monoclonal antibody group was significantly lower than that in the PBMC+SAA group(P＜0.01).Conclusion Anti-CCL20 monoclonal antibod-y can effectively inhibit the expression of pro-inflammatory cytokines in sarcoidosis PBMCs with elevated levels of SAA.
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