The comparative research between hypoxia and dimethyloxalylblycine on the adipogenic differentiation of BMSCs in mouse
Objective To investigate the effects of BMSCs on adipogenesis between that in hypoxia environment and that in normal oxygen environment with hypoxia mimetic agents dimeth-yloxalylglycine(DMOG).Methods BMSCs from the bone marrow mononuclear cells were identified by flow cytometer and osteogenic,adipogenic,chondrogenic differentiation.ELISA was employed to determine the expression of HIF-1α protein in control group(under 21％normal oxygen environment),hypoxia group(under 1％oxygen environment)and DMOG group(under 21％normal oxygen environmentplus 0.5 mmol/LDMOG).Red oil O staining was employed to observe the formation of lipid droplets in BMSCs in each group.The mRNA and protein level of PPAR-γ and C/EBPα were detected using qPCR and Western-blotting in each group.Results Flow cytometry assay showed the bone marrow mononuclear cells were positively expressed with CD90,CD44,CD 105 and negatively expressed with CD34.The cells un-derwent osteogenesis,adipogenesis,chondrogenesis.Compared with the protein expression of the HIF-1α(85.00±1.87)in control group,the protein expression of HIF-1α in hypoxia group and DMOG group(480.4±6.91,331±7.78)were significantly upregulated(P＜0.01).Compared with that in DMOG group,the protein expression of HIF-1α in hypoxia group was significantly upregulated(P＜0.01).To compare with the positive cells(51.80±4.15)in control group,the formation of lipid droplets in hypoxia group and DMOG group(27.80±3.11,31.20±2.59)were significantly decreased(P＜0.05).No significant difference was observed on the forma-tion of lipid droplets between that in hypoxia group and that in DMOG group(P＞0.05).Compared with control group,the mRNA expres-sion of PPAR-y and C/EBPα in hypoxia group and DMOG group were significantly decreased(P＜0.01).Compared with hypoxia group,the mRNA expression of PPAR-γ and C/EBPα in DMOG group were significantly decreased(P＜0.05).Compared with control group,the protein expression of PPAR-γ in hypoxia group and DMOG group were decreased 28.3％and 39.8％(P＜0.01).The protein expres-sion of C/EBPα in hypoxia group and DMOG group were decreased 36.7％and 42.5％(P＜0.01).Compared with hypoxia group,the protein expression of PPAR-γ and C/EBPα in DMOG group were decreased 16％and 9.1％(P＜0.05).Conclusion 1％physical hy-poxia and hypoxia mimetic agents DMOG can both inhibit the BMSCs adipogenesis,whereas 1％physical hypoxia had more powerful inhibitory effect than the DMOG.
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