Cognitive improvement mechanism of aerobic exercise via Keap1Nrf2H0-1 pathway in sleep-disordered aged rats
Objective To explore the cognitive improvement mechanism of aerobic exercise in elderly rats with sleep disor-ders through Keap1Nrf2HO-1 pathway.Methods 60 Wistar rats(30 male and 30 female rats)were divided into 4 groups,the blank group,model group,drug group(according to the qualiy of each rat's body shall be caried out in accordance with the 0.5 mg/kg estazol-am lavage administration)and aerobic exercise group.Each group con-sisted of 15 rats.Results Compared with the blank group,the es-cape latency of the model group was prolonged(P＜0.01),accompa-nied by a lower number of platform searches,a lower number of tra-versals(P＜0.01),and a lower percentage of the total swimming time spent on the original platform(P＜0.05);while the escape latency of the drug-administered group and the aerobic group was shortened(P＜0.01),accompanied by an increase in the number of platform searches,an increase in the number of traversals(P＜0.01),and a higher percentage of the total swimming time spent on the original platform(P＜0.05).The escape latency was shorter(P＜0.01)in the drug administration group and the aerobic exercise group,and was accompanied by a significant increase in the number of platform searches,an increase in the number of crossings of the original plat-form(P＜0.01),and an increase in the percentage of the total swimming time spent on the original platform(P＜0.05).Compared with the blank group,the model group showed higher MDA level,lower SOD activity(P＜0.01),higher Keapl level(P＜0.01),and lower Nrf2 and HO-1 levels(P＜0.01),while the drug-administered and aerobic exercise groups showed lower MDA level,higher SOD activi-ty(P＜0.01),lower Keap1 level,and higher Nr2 and HO-1 levels(P＜0.01).-1levels were elevated(P＜0.01).Conclusion Aero-bic exercise can improve the cognitive function of rats with insomnia disorder,which maybe related to the antioxidant emergency effect of aerobic exercise by upregulatingthe expression levels of Keap1/Nrf2/HO-1 protein in the hippocampus of rats with sleep disorder.
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