The effects of dihydromyricetin on necroptosis in high glucose-stimulated cardiomyocytes
Objective To investigate the effects of dihydromyricetin in high glucose-stimulated cardiomyocytes injury.Methods Primary cardiomyocytes were pretreated with DHY(80 μmol/L)for 4 h,followed by normal glucose(NG,5.5 mmol/L)or high glucose(HG,33.3 mmol/L)stimulation for 48 h.Subsequently,cell viability was assessed by MTT.The degree of cell damage was evaluated by lactate dehydrogenase(LDH)measurement.The level of oxidative stress was assessed with dihydroethidium(DHE)and MitoSOX staining.The protein expression of receptor interacting protein kinase 3(RIPK3)was detected by immunofluorescence and Western blot.The degree of cardiomyocytes apoptosis was determined by TdT-mediated dUTP nick end labeling(TUNEL).Results Compared with HG group,DHY significantly enhanced cardiomyocytes activity(P=0.003),reduced LDH release(P=0.001),alleviat-ed the fluorescence intensity of DHE and MitoSOX staining,inhibited RIPK3 protein expression(P＜0.01),and reduced the number of positive cells with TUNEL staining(P＜0.01)in high glucose-stimulated cardiomyocytes.Conclusion DHY increased cardiomyo-cytes viability and attenuated cell injury,which might be related to oxidative stress inhibition and necroptosis attenuating in high glu-cose-stimulated cardiomyocytes.
dihydromyricetinhigh glucoseoxidative stressreceptor interacting protein kinase 3
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