Value of serum lncRNA DUXAP8 and miR-24-3p in the diagnosis of preeclampsia and the evaluation of pregnancy outcomes
Objective To analyze the value of serum long non-coding RNA dual homeobox A pseudogene 8(lncRNA DUXAP8)and microRNA(miR)-24-3p in the diagnosis of preeclampsia(PE)and the evaluation of pregnancy outcomes.Methods From March 2019 to September 2022,124 PE patients in the Department of Obstetrics and Gynecology of Chengde City Central Hospi-tal of Hebei Province were collected as the PE group,and meanwhile 124 healthy pregnant women were included as the control group.According to pregnancy outcomes,they were categorized into an ad-verse-outcome group of 27 cases and a good-outcome group of 97 cas-es.qRT-PCR method was applied to determine serum lncRNA DUXAP8 and miR-24-3p levels.Multivariate Logistic regression was applied to analyze the influencing factors of pregnancy outcomes in PE pregnant women.ROC curve was applied to analyze the diagnostic and prognostic value of serum lncRNA DUXAP8 and miR-24-3p lev-els in PE.Results The serum lncRNA DUXAP8 level in the PE group was lower than that in the control group,and the miR-24-3p level was higher than that in the control group(P＜0.05).Target Scan Human website predicted that there may be a targeting relation-ship between lncRNA DUXAP8 and miR-24-3p.Correlation analysis showed a negative correlation between lncRNA DUXAP8 and miR-24-3p(r=-0.471,P＜0.001).The AUC of serum lncRNA DUXAP8 combined miR-24-3p in the diagnosis for PE was obviously higher than that of single diagnosis(ZlncRNA DUAP8-combination=3.285,P=0.001,ZmiR-24-3p-combination=3.020,P=0.003),and the sensitivity of combined diagnosis was 92.74％,and the specificity was 72.31％.The serum lncRNA DUXAP8 level in the good-outcome group was higher than that in the adverse-outcome group,and the miR-24-3p level was lower than that in the adverse-outcome group(P＜0.05).The systolic blood pressure,diastolic blood pressure,prenatal blood glucose,hemoglobin,24-hour urine protein,white blood cell count,and LDH in the good-outcome group were lower than those in the adverse-outcome group,while platelet count and serum albu-min were higher than those in the adverse-outcome group(P＜0.05).LncRNA DUXAP8 was a protective factor against adverse pregnan-cy outcomes in PE pregnant women,while miR-24-3p was a risk factor(P＜0.05).The AUC of serum lncRNA DUXAP8 combined miR-24-3p in the diagnosis in pregnant women with PE was obviously higher than that of single diagnosis(ZlncRNA DUXAP8-combination=2.113,P=0.035,ZmiR-24-3p-combination=3.033,P=0.002),and the sensitivity of combined diagnosis was 88.89％,and the specificity was 80.41％.Conclusion Reduced levels of lncRNA DUXAP8 and elevated levels of miR-24-3p in pregnant women with PE may be associated with the development of PE and maternal pregnancy outcomes.
long non-coding RNA dual homeobox A pseudogene 8micro RNA-24-3ppreeclampsiapregnancy outcome
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