Optimization of COPD rat modeling time based on model evaluation
Objective To explore the key indicators for evaluating the rat model of chronic obstructive pulmonary disease(COPD)and optimize the modeling time in light of the current reports on the construction of COPD rat models by cigarette smoking(CS)combined with airway infusion of lipopolysaccharide(LPS).Methods The COPD rat model was replicated by CS combined with airway infusion of LPS.The rats were divided into 4-week blank group,8-week blank group,12-week blank group,4-week model group,8-week model group and 12-week model group according to the smoking duration.The general condition and body weight of the rats were observed and recorded.The lung function of rats was measured by small animal lung function apparatus.The expression lev-els of inflammatory factors tumor necrosis factor-α(TNF-α)and inter-leukin-6(IL-6)in alveolar lavage fluid of rats were detected by en-zyme-linked immunosorbent assay.Results After the modeling,the body weight of rats in 4,8 and 12 week blank group was signifi-cantly higher than that in 4,8 and 12 model week group,respectively(P<0.05).The pathological results of the 4-week model group showed inflammatory cell infiltration;The pathological results of the 8-week model group showed inflammatory cell infiltration,partial alveolar sep-tum rupture and alveolar fusion.The pathological results of the 12-week model group showed inflammatory cell infiltration,a large number of alveolar septa fractures,and alveolar fusion.Compared with 8-week blank group,FEV0.3/FVC%in 8-week model group was significantly decreased(P<0.05);FEV0.1/FVC%and FEV0.3/FVC%in 12-week model group were significantly lower than those in 12-week blank group(P<0.05).There were no significant differences in FEV0.1/FVC and FEV0.3/FVC between the 4-week blank group and 4-week model group(P>0.05).TNF-α and IL-6 in 4,8 and 12 week model group were higher than those in 4,8 and 12 week blank group,respectively(P<0.05).Conclusion Lung function is the key indicator in evaluating COPD rat model,and single inflammatory indicator is not the mark of successful model building.CS ex-posure for 12 weeks combined with airway infusion of LPS can establish a more clinically appropriate COPD rat model.
chronic obstructive pulmonary diseaserat modellung functionmodeling time
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