Experimental study on the application of coacervate adhesive to bone bonding and bone repair in a wet en-vironment
Objective To use polyvinyl alcohol,tannic acid and hydroxyapatite to develop a new type of coacervate adhesive with the ability of bone bonding and bone repair in wet environment and to verify its effect.Methods Using cyanoacrylate adhesive as control group,two new coacervate adhesive were prepared,charac-terized by infrared spectroscopy,and the bonding effect of the three adhesives in wet environment was measured.Their effects on MC3T3-E1 cells were evaluated by CCK-8 method and live staining.They were implanted into the rabbit femur defect,and their bone repair abil-ity was verified by microCT in vivo.Results Two kinds of coacer-vate adhesives,PVA@TA and PVA@TA@HA,were successfully con-structed,and the effectiveness of the synthesis was confirmed by infra-red spectrum analysis.The underwater bonding strength[(70.2±2.1)kPa,(63.3±2.6)kPa]of the two coacervate adhesives in wet envi-ronment was higher than that of cyanoacrylate[(22.6±3.8)kPa],and the difference was statistically significant(P<0.05).The prolifer-ation of MC3T3-E1 cells under the conditions of PVA@TA,PVA@TA@HA adhesive extract was better than that of cyanoacrylate adhe-sive extract by CCK-8 method and live staining.Quantitative microCT analysis proved that after implantation of PVA@TA@HA adhe-sive into bone defects,the growth of new bone was better than that of the other two groups,showing better bone repair promoting abili-ty.Conclusion The coacervate adhesive based on polyvinyl alcohol,tannic acid and hydroxyapatite has been successfully construct-ed.Compared with the traditional cyanoacrylate adhesive,it has better performance of bone bonding and promoting bone repair in wet environment.
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