Influences of barium titanate piezoelectric coated porous titanium alloy materials on macrophage prolifera-tion and osteoclast differentiation
Objective To investigate the influences of barium titanate piezoelectric coated porous titanium alloy materials on macrophage proliferation and osteoclast differentiation.Methods Barium titanate coated porous titanium alloy(PTB)and pure po-rous titanium alloy(PT)were used as experimental group and control group,respectively.The surface characteristics of PTB were ob-served by micro-CT,SEM and EDS.The material extract was prepared by a cyclic loading device,and the RAW264.7 cell-material co-culture was carried out under dynamic loading conditions,and the growth state and proliferation activity of RAW264.7 cells were ob-served by SEM,CCK-8 and AM-PI staining.RANKL was used to induce osteoclast differentiation and TRAP staining of cells cocul-tured with PTB.The effect of PTB on osteoclast differentiation was ob-served and analyzed.Results Micro-CT showed that the coating had little effect on the overall structure,SEM showed that the surface barium titanate coating was dense,regular and tightly bound,EDS showed that the composition of Barium titanate coating elements was reasonable and evenly distributed,and material piezoelectric coeffi-cient(d33)measurement showed that the piezoelectric coefficient of PTB group was close to that of natural bone.CCK-8 showed that the cytotoxicity level of the dynamic extract of materials in the two groups was grade Ⅰ,SEM showed that the surface cells of the two groups were in a good state of growth,Calcin-AM/PI staining showed that the cells in the PTB group showed stronger proliferation activ-ity,and flow apoptosis showed no statistical significance between the two groups and the blank control group(P>0.05).The results of osteoclast induction showed that the total number of cells in PTB group was higher,and the number and proportion of osteoclasts were decreased.Conclusion PTB can promote cell proliferation and inhibit osteoclast differentiation by forming local micro electric field under loading conditions.
3D printingtitanium alloybarium titanateRaw264.7 cellsosteoclast induction
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