Expression of E2F8 in human colorectal cancer and its relationship with the prognosis of patients
Objective To investigate the expression of adenovirus E2 promoter binding factor 8(E2F8)in colorectal cancer(CRC)and its effect on the prognosis of colorectal cancer patients.Methods The mRNA expression levels of E2F8 in colorectal cancer were analyzed in Cancer Genome Atlas(TCGA)database and Gene Tissue Expression(GTEx)database.The expression of E2F8 in colorectal cancer tissues and cells was detected by real-time fluores-cence quantitative PCR(qPCR)and Western blot.Immunohisto-chemical staining was used to analyze the relationship between E2F8 protein expression and clinical features and prognosis of 98 patients with colorectal cancer.COX proportional risk model was used to ana-lyze the prognostic factors of CRC.Results The expression level of E2F8 in tumor tissues was higher than that in normal tissues in TCGA database and clinical samples.E2F8 expression levels in colorectal cancer cell lines were higher than normal colon epithelial cell lines except HCT116 and HCT15.The overall survival,disease-free survival and metastasis-free survival of patients with high ex-pression of E2F8 were shorter than those with low expression of E2F8(all P<0.01),and the Dukes stage of colorectal cancer tissues with high expression of E2F8 was later(P<0.05).Univariate COX regression model analysis showed that women(HR=2.009,95%CI:1.139-3.544),lymph node invasion(HR=1.780,95%CI:1.005-3.150),Dukes C/D stage(HR=2.538,95%CI:1.393-4.623),TNM III/IV(HR=2.894,95%CI:1.554-5.392),and high expression of E2F8(HR=2.807,95%CI:1.540-5.118)were associated with poor prognosis in colorectal cancer patients(all P<0.05).Multivariate COX regression model analysis showed that high expression of E2F8(HR=2.747,95%CI:1.447-5.216)and late TNM stage(HR=5.543,95%CI:1.537-19.990)were independent risk factors for poor prognosis in pa-tients with colorectal tumors(all P<0.05).Conclusion E2F8 is highly expressed in colorectal cancer,and high expression of E2F8 in colorectal cancer tissues is associated with late Dukes staging and poor prognosis in colorectal cancer patients.High expression of E2F8 is an independent risk factor for poor prognosis in colorectal cancer patients,and E2F8 can be used as a potential marker for dis-ease progression and prognosis in colorectal cancer patients.
NETL
NSTL
万方数据
