Effect of miR-760/CPEB2 axis on apoptosis and proliferation in acute lymphoblastic leukemia
Objective This study was aimed to explore the effect and mechanism of miR-760 on apoptosis and proliferation of lymphoblasts in acute lymphoblastic leukemia(ALL).Methods Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the relative expression of miR-760 and cytoplasmic polyadenylation element binding protein 2(CPEB2).Human B-lymphocyte acute leukemia cells Ball-1 were transfected with miR-NC,miR-760 mimic,si-NC,si-CPEB2,oe-NC,oe-CPEB2,miR-760 mimic+oe-NC and miR-760 mimic+oe-CPEB2,respectively=.Cell apoptosis was examined by flow cytometry.Relative expression of Bax,c-caspase-3,t-caspase-3,Ki67,PCNA and CPEB2 was detected by Western blot-ting.The targeting relationship of miR-760 and CPEB2 was verified by luciferase report experiment.Results Compared with human B lymphoblasts HMy2.CIR cells,miR-760 expression was decreased in ALL cell lines Ball-1,Reh and JurkatE6,while CPEB2 mRNA and protein expression was increased(P<0.05).Compared with miR-NC group,apoptosis rate and protein expression of Bax in miR-760 mimic group was increased,while Ki67 and PCNA expression was de-creased(P<0.05).Bioinformatics methods predicted that miR-760 targeted CPEB2.Dual-luciferase reporter assay verified that CPEB2 was the target of miR-760.Compared with si-NC group,the apoptosis rate,Bax and c-caspase-3 expression were increased,while pro-tein expression of Ki67 and PCNA was decreased in si-CPEB2 group(P<0.05).Up-regulation of CPEB2 reversed the promotion of miR-760 overexpression on cell apoptosis rate,Bax and c-caspase-3 expression,as well as its inhibition on protein expression of Ki67 and PCNA(P<0.05).Conclusion MiR-760 targets CPEB2 to activate cell apoptosis and inhibit cell proliferation,which provides a new option for ALL treatment.
miR-760acute lymphocytic leukemiaapoptosisproliferationcytoplasmic polyadenylation element binding protein 2
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