The causal relationship between specific gut microbiota and osteoporosis
Objective To investigate the causal relationship between the gut microbiota and the risk of osteoporosis by adopt-ing Mendelian randomization(MR)analysis approach.Methods The data was obtained from a genome-wide association study(GWAS)on the gut microbiota and osteoporosis.The single nucleotide polymorphism(SNP)was used as instrumental variables and SNPs that were sensitive to the gut microbiota were selected for analysis.Two-sample MR analyses were performed using IVW,WM,and MR-Egger regression methods to estimate the causal relation-ship between the gut microbiota and osteoporosis,with odds ratios(OR)as the effect size estimates.Sensitivity and pleiotropy tests were conducted to evaluate the robustness and potential bias of the results.Results A total of 1531 SNPs were included as instru-mental variables in this study.The Mendelian randomization analy-sis revealed a causal relationship between Clostridiumsensustric-to1,Coprococcus 3,Faecalibacterium,Fusicatenibacter,Phasco-larctobacterium,Ruminococcaceae NK4A214 group,Sellimonas,and osteoporosis.The odds ratios(OR)estimated by the random-ef-fects model IVW method were 1.291(95%CI 1.037-1.607,P=0.022),0.627(95%CI 0.440-0.894,P=0.010),1.520(95%CI 1.125-2.054,P=0.006),1.271(95%CI 1.037-1.558,P=0.021),0.801(95%CI 0.674-0.951,P=0.012),1.220(95%CI 1.010-1.473,P=0.039),and 0.854(95%CI 0.744-0.979,P=0.024),respectively.These results indicate a causal relationship between the gut microbi-ota and osteoporosis.The Cochran Q test for IVW(P>0.05)and MR-Egger regression(P>0.05)indicated no heterogeneity among the SNPs.The MR-Egger method showed that the egger-intercept was not significantly different from zero(P>0.05),suggesting no horizon-tal pleiotropy among the SNPs.Conclusion This study suggests a causal relationship between seven bacterial genera(Clostridium-sensustricto1,Coprococcus3,Faecalibacterium,Fusicatenibacter,Phascolarctobacterium,Ruminococcaceae NK4A214 group,Sell-imonas)and osteoporosis,providing new insights into the pathogenesis of intestinal microbiota mediated osteoporosis.
Mendelian randomizationgut microbiotaosteoporosisgenome-wide association studycausal relationship
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万方数据
