Effects of long non-coding RNA RNF157-AS1 on malignant biological behavior of lung adenocarcinoma cells
Objective To investigate the effects of long non-coding RNA RNF157-AS1 on the malignant biological behavior of lung adenocarcinoma cells through cell experiments.Methods Quantitative Real-time fluorescent PCR(qRT-PCR)was used to detect the expression of RNF157-AS1 in lung adenocarcinoma cell lines(H1299,A549,PC9 and Calu3)and normal lung epithelial cells(HBE).Small interfering RNA was transfected into lung adenocarcinoma cells to knock down the expression of RNF157-AS1,and the interfering efficiency was verified by qRT-PCR.After successfully interfering with RNF157-AS1 in two lung adenocarcinoma cell lines,they were divided into control group(NC group)and interference group(si l group,si2 group).The effects of RNF157-AS1 on the proliferation,migration and invasion of lung adenocarcinoma cells were investigated by CCK8 assay,Transwell migration assay and Matrigel invasion assay,respectively.Results Compared with HBE,the expression of RNF157-AS1 in 4 lung adenocarcinoma cell lines was significantly up-regulated(P＜0.01 for all),and H1299 and PC9 cell lines with the highest expression levels were selected as the follow-up test cell lines.qRT-PCR confirmed that the two small inter-fering RNAs could effectively knock down the expression of RNF157-AS1,and the interference efficiency was more than 70％(P＜0.01).CCK8 assay showed that,compared with the control group,the prolif-eration ability of cells was significantly decreased after interference of RNF157-AS1 expression(P＜0.01).The Transwell assay showed that,compared with the control group,the migration ability of RNF157-AS1 cells was significantly decreased after interference(P＜0.01).Matrigel's experiment showed that interference with RNF157-AS1 significantly reduced the invasive ability of cells compared with the control group(P＜0.01).Conclusion RNF157-AS1 is highly ex-pressed in lung adenocarcinoma cells,and affects the proliferation,migration and invasion of lung adenocarcinoma cells.
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