Effect of silencing circ_0000502 on biological behavior of multiple myeloma cells
Objective To investigate the effect of silencing circ_0000502 on the biological behavior of multiple myeloma cells and its possible mechanism.Methods The qRT-PCR method was used to detect the expression levels of circ_0000502 and miR-338-3p in serum of patients with multiple myeloma and healthy subjects(control group).In vitro culture of human multiple myelo-ma cells RPMI-8226,si-NC,si-circ_0000502,miR-NC,miR-338-3p mimic,si-circ_0000502 and miR-338-3p inhibitor were transfect-ed into RPMI-8226 cells,respectively.They were classified as si-NC group,si-circ_0000502 group,miR-NC group,miR-338-3p mimic group,si-circ_0000502+miR-338-3p inhibitor group,and normal cultured cells were taken as normal culture group.CCK-8 experiment was used to detect cell proliferation.Flow cytometry was used to detect apoptosis rate.Scratch test was used to detect cell migration ability.The targeting relationship between circ_0000502 and miR-338-3p was verified.Western blot method was used to detect cleaved-caspase3 protein expression.Results Compared with the control group,the expression of circ_0000502(1.01±0.15)and miR-338-3p(1.03±0.12)was increased in multiple myeloma patients(5.39±0.26).The expression level of miR-338-3p was decreased(0.25±0.05)(P＜0.05).Silencing circ_0000502 or overexpression of miR-338-3p decreased cell viability and scratch healing rate,and increased apop-tosis rate and cleaved caspase3 protein levels(P＜0.05).circ_0000502 could target miR-338-3p and negatively regulate the expression of miR-338-3p.Down-regulation of miR-338-3p reduced the effects of circ_0000502 on RPMI-8226 cell viability,scratch healing rate,apoptosis rate,and Cleaved caspase3 protein levels(P＜0.05).Conclusion Silencing circ_0000502 can inhibit the proliferation and migration of multiple myeloma cells and induce cell apoptosis by targeting miR-338-3p regulation.
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