Correlation between macrophage polarization and CXCL9 and the progression and recurrence of inflam-matory bowel disease
Objective To explore the correlation between the ratio of M1/M2 macrophages and CXCL9 and the progression and recurrence of inflammatory bowel disease(IBD).Methods A total of 70 IBD patients admitted to the Second Hospital affiliated to Jiax-ing University from September 2021 to June 2023 were selected.Dur-ing the same period,30 gender and age-matched healthy individuals undergoing physical examinations were selected as controls.They were divided into the following groups:control,primary active,remis-sion,and relapsed active.Serum samples from all four groups were tested and compared for TNF-α,INOS,IL-6,IL-10,ARG-1 and CXCL9 using enzyme-linked immunosorbent assay(ELIS A).HE staining and immunohistochemistry were used to examine intestinal tissue in-filtration by inflammatory cells,the specific markers for M1 macrophages(CD86),M2 macrophages(CD206)and the chemokine CX-CL9 across the four groups.Logistic regression analysis was performed to identify influencing factors in the serum.The INOS/ARG-1 ra-tio represented the proportion of M1/M2 macrophages.Receiver operating characteristic(ROC)curves were plotted for different indica-tors to assess predictive values for IBD progression and recurrence.Results The levels of TNF-α,INOS,IL-6,IFN-γ cytokines asso-ciated with M1 macrophages were significantly higher in the primary and relapsed active groups compared to the control and remission groups(P＜0.05);The levels of ARG1 and IL-10 associated with M2 macrophages were significantly higher in the remission group com-pared to the control,primary and relapse active group(P＜0.05);Compared to the control and remission group,the M1/M2 ratio and CXCL9 levels were significantly elevated in the primary and relapse active groups(P＜0.05).TNF-α,INOS,IL-6,IFN-γ,M1/M2 ratio,and CXCL 9 were identified as influential factors for assessing disease activity in the primary active group(P＜0.05).ARG 1 and IL-10 were influencing factors for assessing the disease activity of in the remission group(P＜0.05).TNF-α,INOS,IL-6,IFN-γ,M1/M2 ratio,and CXCL9 were identified as influencing factors for assessing disease activity in the relapsed active group(P＜0.05).The combined assessment of M1/M2 ratio and CXCL9 has an AUC of 0.937(95％CI:0.891-0.982,P＜0.05)for evaluating IBD disease activity.Based on the Youden index,the optimal cut-off values for IBD disease activity were 2.410 and 396.57,respectively,providing a sensitivity of 91.4％and specificity of 91.2％.Conclusion The M1/M2 macrophage ratio and CXCL9 levels are correlated with the IBD progres-sion and recurrence,and have certain predictive value for assessing the disease activity of IBD.
NETL
NSTL
万方数据
