异种肝肾联合移植中猪内源性逆转录病毒的检测
Detection of porcine endogenous retrovirus in combined liver and kidney xenotransplantation
张虹 1张玄 1陶开山 1窦科峰1
作者信息
- 1. 空军军医大学西京医院肝胆外科,陕西西安 710032
- 折叠
摘要
目的 检测猪内源性逆转录病毒(PERV)基因在异种肝肾联合移植供受体中的表达,以期为PERV的跨物种传播提供参考依据.方法 以6-基因编辑猪-恒河猴肝肾联合移植的组织样本作为研究对象,采用RT-PCR和PCR技术检测移植前后供受体不同组织中PERV编码基因、PERV各亚型以及猪线粒体细胞色素B基因的表达情况.结果 供体猪的不同组织中存在PERV编码基因gag和pol、PERV-A和PERV-B亚型以及猪线粒体细胞色素B基因,但不存在PERV-C亚型.移植终点时受体不同组织中均未检出PERV基因序列.结论 基因编辑猪-恒河猴异种肝肾联合移植受体存活的14 d内没有发生PERV的跨物种传播.
Abstract
Objective To detect the expression of porcine endogenous retrovirus(PERV)genes in donor and recipient of combined liver and kidney xenotransplantation,hoping to provide a reference for the cross-species transmission of PERV.Methods The tissue samples of 6-gene-edited pig-to-rhesus macaque combined liver and kidney xenotransplantation were selected as research objects.The expressions of PERV coding gene,PERV subtypes,and porcine mitochondrial cytochrome B gene in different tissues of donor and recipient samples during xenotransplantation were detected by RT-PCR and PCR.Results There were PERV coding genes gag and pol,PERV-A and PERV-B subtypes,and porcine mitochondrial cytochrome B gene in different tissues of donor pig,but no PERV-C subtype was detected in the donor tissue samples.At the end of transplantation,no PERV gene sequences were detected in different tissues of the recipient.Conclusion No cross-species transmission of PERV occurred within 14 d of survival in gene-edited pig-to-rhesus macaque combined liver and kidney xenotransplantation.
关键词
异种器官移植/猪内源性逆转录病毒/人畜共患病/跨物种传播Key words
xenotransplantation/porcine endogenous retrovirus/zoonotic diseases/cross-species transmission引用本文复制引用
基金项目
国家自然科学基金(82070671)
国家自然科学基金(82371793)
国家自然科学基金(82170667)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据