Objective To investigate the effects of simulated microgravity on embryonic development and gene expression alterations in Bombyx mori.Methods Bombyx mori embryos treated in simulated microgravity with 3D clinostat were used as treatment group.The control group consisted of untreated Bombyx mori embryos from the same batch.Transcriptomic sequencing was conducted on randomly selected samples of Bombyx mori embryos or newly-hatched larvae from both the control and treatment groups.Embryonic development status and larval hatching time of Bombyx mori were observed,and the differentially expressed genes in the transcriptome were analyzed.The signaling pathways involved in differentially expressed genes were investigated through GO and KEGG enrichment analysis,and the expression of these differential genes was validated by qRT-PCR.Results Simulated microgravity advanced the development of Bombyx mori embryos.GO enrichment analysis revealed that,compared with the control group,differentially expressed genes of embryos and newly-hatched larvae in the treatment group were primarily enriched in biological processes related to catalytic activity,membrane structure,and metabolism.KEGG pathway enrichment analysis showed that,compared with the control group,differentially expressed genes of embryos in the treatment group were primarily enriched in ribosome and RNA transport pathways,while those of newly-hatched larvae in the treatment group were mainly enriched in metabolism,focal adhesion,and tight junction pathways.qRT-PCR results indicated that,compared with the control group,embryos in the treatment group exhibited significant upregulation of genes such as organic cation transporter protein and synaptic vesicle glycoprotein 2B,and newly-hatched larvae in the treatment group showed significant upregulation of genes such as aminopeptidase N,and significant downregulation of genes such as collagen and calcium-binding EGF domain.Conclusion Simulated microgravity speeds up Bombyx mori embryonic development,and the gene expression of Bombyx mori is sensitive to microgravity.
关键词
模拟失重/家蚕胚胎发育/基因表达
Key words
simulated microgravity/Bombyx mori embryonic development/gene expression
维普
万方数据