Objective To investigate the effect of HOXB7 in gastric intestinal metaplasia(GIM)induced by bile acid.Methods qRT-PCR and Western blotting were used to detect the expression levels of HOXB7 and several intestinal markers in different cell lines and mucosal tissues.HOXB7 overexpressing and knock-down cell models were established with overexpressing plasmid and specific siRNA transfection,and the expression changes of intestinal markers were detected.Results In gastric epithelial cell GES-1,deoxycholic acid induced the up-regulation of the expression of HOXB7 and intestinal markers such as CDX2 and MUC2 in a time-dependent and concentration-dependent manner(P<0.01).The expression level of HOXB7 was significantly higher in several gastric cancer cell lines and normal intestinal mucosal cell lines than that in GES-1(P<0.01).The expression level of HOXB7 in mouse large intestinal mucosa was significantly higher than that in esophageal and gastric mucosa(P<0.05),showing an expression pattern along the anterior-posterior axis.The expression level of HOXB7 was significantly higher in GIM tissues of GIM patients than that in paired normal mucosa(P<0.01).HOXB7 could regulate the expression of intestinal markers such as CDX2,KLF4 and MUC2 in gastric epithelial cell lines(P<0.05,P<0.01),while knock-down of HOXB7 could reduce the expression of intestinal markers induced by deoxycholic acid(P<0.01).Conclusion HOXB7 is involved in the induction and regulation of GIM induced by bile acid.In-depth understanding of the upstream and downstream molecular pathways of HOXB7 is helpful to find new targets for the prevention and treatment of GIM.
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