Objective To elucidate the biological function of Wilms'tumor 1-associating protein(WTAP)in colorectal cancer cell differentiation,and to explore the molecular mechanism of WTAP affecting colorectal cancer cell differentiation.Methods The expression patterns of WTAP in colorectal cancer cell differentiation were detected by cell differentiation model.Stably transfected cell lines with overexpression or knockdown of WTAP were constructed,and the cell differentiation indexes were detected by qRT-PCR and Western blotting to analyze the effects of WTAP on the differentiation status of colorectal cancer cells.Transcriptomics was used to explore the molecular mechanism of WTAP on colorectal cancer cell differentiation.Results The cell differentiation model showed that the expression level of WTAP was significantly down-regulated with colorectal cancer cell differentiation.Overexpression of WTAP could inhibit colorectal cancer cell differentiation,while knockdown of WTAP could accelerate colorectal cancer cell differentiation.The transcriptomics suggested that the glycolytic pathway was significantly abnormal in colorectal cancer cell differentiation.Further molecular biological experiments verified that WTAP could promote the expression of glycolysis-related enzymes in colorectal cancer cells.Conclusion WTAP inhibits cell differentiation by promoting glycolysis in colorectal cancer cells.
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