Objective To explore the relationship between the degree of immune cell infiltration and the prognosis of colorectal mucinous adenocarcinoma(MAC),and to provide theoretical support for early clinical decision-making.Methods Gene expression profiles and clinical prognosis information of patients with colorectal MAC were collected from The Cancer Genome Atlas database.The CIBERSORT algorithm was used to quantify the composition ratio of 22 tumor-infiltrating immune cells(TIICs).Kaplan-Meier survival analysis,LASSO regression,and Cox univariate and multivariate regression were performed to identify independent prognostic factors for MAC.The correlation between TIICs and clinical staging was analyzed using rank sum test.Results The results of multivariate Cox regression analysis showed that the degree of tumor infiltration of regulatory T cells(Tregs;P =0.000 8),M0 macrophages(P =0.000 6),and activated dendritic cells(DC;P =0.002 0)was an independent risk factor for the prognosis of MAC patients.In addition,with malignant progression of clinical staging,the degree of infiltration of M0 macrophages gradually increased(P =0.004 5),and the degree of infiltration of CD8+ T cells decreased gradually(P =0.005 5).In tumor tissues with distant metastasis,the degree of infiltration of CD8+T cells(P =0.041 3)and activated DC(P =0.037 7)was lower than that in patients without metastasis.Conclusion TIICs are associated with the prognosis and malignant progression of colorectal MAC.Tregs,M0 macrophages,and activated DC are all negatively correlated with the prognosis.
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