Objective To investigate the aging rule of aquaporin-1(AQP1)expression level in lung tissue in acute respiratory distress syndrome(ARDS),and its correlation with the degree of lung injury and classic inflammatory indicators,so as to find novel biomarkers and intervention targets for ARDS.Methods A mouse model of ARDS was prepared by intranasal instillation of lipopolysaccharide(LPS).The body mass of the mice was recorded daily,and lung tissue and bronchoalveolar lavage fluid were collected at 6 h,1 d,3 d,and 7 d.HE was used to analyze the pathological changes in lung tissue.ELISA and qPCR were used to detect the levels of inflammatory factors IL-1 β,IL-6,and TNF-α.Flow cytometry was used to analyze the number and proportion of innate immune cells,such as neutrophils,alveolar macrophages,eosinophils,and monocytes.qPCR and Western blotting were used to detect AQP1 mRNA and protein expression levels.Pearson's method was used to analyze the correlation between AQP1 and inflammatory cells.Results After LPS stimulation for 6 h,there was inflammatory cell infiltration in the lung tissue of mice,severe lung injury appeared on day 3,and recovered on day 7.The mRNA and protein levels of inflammatory factors IL-1 β,IL-6,and TNF-α increased until day 3 and decreased on day 7(P＜0.05).The proportion of neutrophils was significantly increased and decreased on day 7,while the proportion of alveolar macrophages,eosinophils,and monocytes continued to decrease until day 3 and increased on day 7(P＜0.05).AQP1 mRNA and protein expression levels continuously decreased(P＜0.05).The expression level of AQP1 was significantly correlated with four kinds of inflammatory cells(P＜0.01).Conclusion The expression level of AQP1 in lung tissue in ARDS is significantly negatively correlated with the degree of lung injury and inflammatory indicators,which can be used as a new biomarker of lung injury,and further reveal the possible protective role of AQP1 with potential clinical value.
维普
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