Objective To establish a C57BL/6 mouse model of sweat measurement after hypoxia exposure.Methods Fourteen male C57BL/6 mice were randomly divided into control group(Con group)and hypoxia exposure group(Hyp group).The mice in the Hyp group were put into the compound environment simulation experimental chamber and exposed to hypoxia for 3 weeks.Then pilocarpine test and iodine-starch test were performed on the mice in each group,and the sweating area was obtained by analyzing the collected experimental pictures.Six C57BL/6 male mice were selected,the methods of random grouping and modeling were the same as before,and the animal model of hypoxia exposure was established.Transmission electron microscopy was used to observe the effect of hypoxia exposure on sweat gland secretory granules in mice sweat gland tissue.Results Compared with the Con group,the Hyp group had a significant reduction in the sweating area.From 4 min after the pilocarpine test,the sweating area of the two groups was statistically different(P＜0.05).The results of transmission electron microscopy showed that high-density sweat gland secretory granules were observed in the Con group,but not in the Hyp group.Conclusion In this study,a mouse model of sweat measurement after hypoxia exposure is successfully constructed by using hypoxia exposure combined with pilocarpine method,and iodine-starch test is used to quantitatively detect the sweating of mice.The establishment of this model lays a good experimental foundation for further study on the effect of hypoxia exposure on sweat secretion and sweat gland function.
维普
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