Objective To determine an appropriate molecular identification method for Dermacentor marginatus by comparing the gene differences in ribosomal fragments of Dermacentor marginatus in Wuwei City,Gansu Province.Methods In March 2022,128 Dermacentor marginatus were collected from Wuwei City,and 10 were randomly selected for the experiment.First,morphological identification of ticks was performed by a stereomicroscope.Then,ribosome sequences of 5.8S,18S,28S,ITS1 and ITS2 of Dermacentor marginatus were amplified and sequenced.MEGA 11 software was used to construct the phylogenetic tree of tick species,and then DNASTAR software was used to compare the genetic homology of Dermacentor marginatus and other common ticks such as Haemaphysalis longicornis.Results The results of morphological identification showed that all ticks were the same species—Dermacentor marginatus.Phylogenetic relationships showed that ribosome 18S,28S,ITS1 and ITS2 sequences could distinguish ticks of genus Dermacentor from other different species,while 5.8S sequence had no distinguishing ability.Meanwhile,18S and ITS1 sequences could distinguish Dermacentor marginatus from other ticks,and 18S sequence could also distinguish Dermacentor marginatus from different regions.Conclusion The ribosome 18S sequence can distinguish different geographical strains of Dermacentor marginatus and has a strong ability as an effective marker for molecular identification of Dermacentor marginatus.
维普
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