Objective To investigate the diagnostic value of antisense RNA1(ATP1A1-AS1)of serum long non-coding RNA Na+/K+-ATPase A1 subunit on pancreatic cancer and its effect on the proliferation,apoptosis and telomerase activity of pancreatic cancer cells.Methods Sixty-five patients with pancreatic cancer who were treated in Xuancheng People's Hospital from April 2020 to October 2023 were selected as the research subjects,and 60 healthy subjects were selected as controls at the same period.qRT-PCR was used to detect ATP1A1-AS1 expression levels in serum of two groups of patients.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of serum ATP1A1-AS1 on pancreatic cancer.Pancreatic cancer cells PANC-1 were cultured in vitro,and the expression of ATP1A1-AS1 in PANC-1 cells was up-regulated by transfection of ATP1A1-AS1 overexpression vector.MTT was used to detect the effect of up-regulating ATP1A1-AS1 expression on the proliferation of PANC-1 cells.Flow cytometry was used to detect the effect of up-regulating ATP1A1-AS1 expression on the apoptosis of PANC-1 cells.Western blotting was used to detect the effect of up-regulating ATP1A1-AS1 expression on the expression of CyclinD1,Ki-67,B-cell lymphoma-2(Bcl-2)and Bcl-2-associated X protein(Bax)in PANC-1 cells.Telomerase repeat amplification protocol was used to detect the effect of up-regulating ATP1A1-AS1 expression on the telomerase activity of PANC-1 cells.Results Compared with the control group,the expression level of ATP1A1-AS1 in the serum of patients with pancreatic cancer was significantly reduced(P＜0.05).The results of ROC curve analysis showed that the sensitivity of serum ATP1A1-AS1 in the diagnosis of pancreatic cancer was 83.08％,the specificity was 86.67％,and the area under the ROC curve was 0.900.After up-regulating ATP1A1-AS1 expression,the proliferation ability,telomerase activity,and the expression levels of CyclinD1,Ki-67,and Bcl-2 in PANC-1 cells were reduced(P＜0.05),while the apoptosis rate and Bax protein expression level were increased(P＜0.05).Conclusion ATP1A1-AS1 is low expressed in the serum of patients with pancreatic cancer and may be a potential biomarker for the diagnosis of pancreatic cancer.Up-regulating ATP1A1-AS1 can inhibit the proliferation and induce apoptosis of pancreatic cancer cells,which may be related to the regulation of CyclinD1,Ki-67,Bcl-2 and Bax protein expression and the inhibition of telomerase activity.
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