Objective To analyze the effects of short-term hypergravity load on adult neurogenesis and spatial working memory in mice.Methods Male C57BL/6 mice were randomly divided into control group,9 G hypergravity centrifugation group(9 G group)and 15 G hypergravity centrifugation group(15 G group),with 8 mice in each group.The mice in the experimental groups were subjected to centrifugal force of 9 G or 15 G by centrifugation for 15 min a day for 3 weeks.Then,behavioral analysis of water maze,eight-arm maze,open field test and elevated plus maze test were performed to compare the differences of spatial learning and working memory,anxiety level and motor function between the groups.Immunofluorescence staining was used to analyze the neurogenesis phenotype of the subventricular zone and the subgranular zone of the hippocampal dentate gyrus.Results Compared with the control group,the body mass of mice in 9 G group and 15 G group decreased significantly after centrifugation(P＜0.05,P＜0.01).There were no significant differences in behavioral indicators among the three groups.Compared with the control group and the 9 G group,the coronal area of lateral ventricle in the 15 G group was significantly reduced(P＜0.01)and the number of CD24+ependymal cells in the lateral ventricle of the 15 G group was significantly decreased(P＜0.01).There was no significant difference in the neurogenesis of the subventricular zone and the subgranular zone of the dentate gyrus in the hippocampus among the three groups.Conclusion Short-term hypergravity load has no significant effect on neurogenesis,spatial learning and working memory,anxiety level and motor function in mice,suggesting that the mammalian brain has strong adaptability and homeostatic maintenance capacity after short-term hypergravity load(such as short-term flight training),and can still cope with such transient changes of gravity environment.On the other hand,the coronal area of lateral ventricle and the number of ependymal cells decreased significantly in the 15 G group,suggesting that high intensity or long-term hypergravity load may damage brain structure and higher brain functions through ventricular defects.
关键词
超重力负荷/神经发生/空间工作记忆/焦虑
Key words
hypergravity load/neurogenesis/spatial working memory/anxiety
维普
万方数据