Objective To analyze the correlation between sperm survival rate and positive rate of sperm germiculture in patients with necrozoospermia.Methods A retrospective analysis was performed on 110 patients diagnosed with necrozoospermia(necrozoospermia group)by eosin staining after semen examination for more than 2 times in Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Tangdu Hospital,Air Force Medical University from March to September 2022.According to the sperm survival rate,the patients were divided into 3 groups:40 cases of mild necrozoospermia(35％＜survival rate ≤58％),42 cases of moderate necrozoospermia(15％＜survival rate ≤35％)and 28 cases of severe necrozoospermia(0％＜survival rate≤15％).In addition,20 men with normal sperm survival rate were used as control group.Semen examination and sperm germiculture were performed on the enrolled patients,and the positive rate of germiculture was counted.Results After sperm germiculture,the positive rates of each group were as follows:19 cases in the mild necrozoospermia group,26 cases in the moderate necrozoospermia group,23 cases in the severe necrozoospermia group and 3 cases in the control group.The positive rate of germiculture in the necrozoospermia group(68/110)was significantly different from that in the control group(3/20)(x2=14.96,P＜0.01).The positive rate of the severe necrozoospermia group(23/28)was significantly higher than that of the mild necrozoospermia group(19/40)(x2=8.37,P＜0.01).However,there was no statistical difference between the moderate necrozoospermia group(26/42)and the severe necrozoospermia group(23/28),as well as the mild necrozoospermia group(19/40)(P＞0.05).In addition,the positive rate of sperm germiculture significantly increased with the decrease of sperm survival rate,and especially when the sperm survival rate was less than 15％,the positive rate of germiculture significantly increased(x2=6.57,P＜0.05).Conclusion There is a correlation between the positive rate of sperm germiculture and sperm survival rate in patients with necrozoospermia,especially when the sperm survival rate is less than 15％,so it is suggested to use sperm germiculture as a routine examination for patients with severe necrozoospermia.
维普
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