Objective To investigate the self-sealing effect of titanium dioxide nanotube coating in deionized water and its effect on the proliferation and adhesion of rat bone marrow mesenchymal stem cells(BMSCs)and mouse mononuclear macrophages(RAW264.7).Methods Nanotubes were prepared on the surface of pure titanium in blank group by anodic oxidation method.Blank titanium sheet was used as the control group,and deionized water-treated titanium dioxide nanotubes for 0,1,3,5 and 7 d were used as the experimental group.The material characterization was observed,and BMSCs and RAW264.7 were incubated on the surfaces of the six groups,respectively.The surface coating and cell morphology of the implants were observed by scanning electron microscopy.Results The self-sealing effect of titanium dioxide nanotube coated anodic oxide film in deionized water was studied by observing the immersion in deionized water.With the extension of soaking time,more particles covered the nanotubes after 3 d.After 5 d,a"garland"-like structure was formed.The needle-like structure completely closed the nanotubes below after 7 d.The results showed that there was an obvious self-sealing process in the initial stage of the anodized film soaking in deionized water,and the changes of porous layer of the anodized film were observed by scanning electron microscopy.Conclusion Deionized water-treated titania nanotubes can affect the formation of new self-sealing structures on the wall of titania nanotubes.The proliferation and early spread of BMSCs and RAW264.7 are inhibited by titania nanotubes in deionized water.
维普
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