Objective To investigate the expression of fatty acid-binding protein 3(FABP3)in clear cell renal cell carcinoma(ccRCC)and its correlation with TNM staging,pathological grading,and prognosis of ccRCC.Methods The study collected 100 cancer tissue samples and adjacent non-cancerous tissue samples from patients who underwent radical surgery for ccRCC at First Affiliated Hospital of Huzhou University.The expression levels of FABP3 in all ccRCC samples were detected using qRT-PCR and IHC.WHO/ISUP grading system was used for pathological grading of ccRCC,with a total of 4 grades:G1,G2,G3,and G4.Based on the TNM staging,samples were categorized into stages Ⅰ,Ⅱ,Ⅲ,andⅣ.The differences in FABP3 expression among various groups and stages were compared,and the correlation between FABP3 expression levels and overall survival(OS)of ccRCC patients was analyzed.Results qRT-PCR and IHC results showed that FABP3 expression in ccRCC tissues was significantly higher than that in adjacent non-cancerous tissues(P＜0.05).In the WHO/ISUP grading system,the expression level of FABP3 in G4 group(0.94±0.08)was significantly higher than that in G1(0.61±0.08),G2(0.72±0.07),and G3(0.82±0.09),and the difference was statistically significant(P＜0.001).In the TNM staging,the expression level of FABP3 in stage Ⅰ(0.64±0.06)was lower than that in stage Ⅱ(0.76±0.03),that in stage Ⅲ(0.84±0.02)was higher than that in stage Ⅱ,and that in stage Ⅳ(0.92±0.04)was significantly higher than that in stage Ⅲ,with statistically significant difference(P＜0.001).The expression level of FABP3 was significantly positively correlated with TNM staging and WHO/ISUP grading of ccRCC(P＜0.05).Cox analysis showed that high expression of FABP3(HR=2.21,95％CI:1.47-2.95,P＜0.001),stage Ⅲ(HR=1.41,95％CI:1.07-2.61,P=0.01)and stage Ⅳ(HR=2.65,95％CI:1.26-3.23,P＜0.001)in TNM staging,and G3(HR=1.25,95％CI:1.05-2.95,P=0.014)and G4(HR=2.87,95％CI:1.54-3.60,P＜0.001)in WHO/ISUP grading were risk factors for poor prognosis in patients with ccRCC.Conclusion In ccRCC patients,FABP3 is highly expressed,and its high expression is related to poor prognosis of ccRCC,which suggests that FABP3 may serve as a potential molecular marker for predicting ccRCC prognosis,providing a theoretical basis for further research into the molecular mechanisms and subsequent treatment strategies of ccRCC.
关键词
脂肪酸结合蛋白3/肾透明细胞癌/WHO/ISUP分级/TNM分期
Key words
fatty acid-binding protein 3/clear cell renal cell carcinoma/WHO/ISUP grading/TNM staging
维普
万方数据