Objective To compare the differences in the range and brain regions between lateral ventricle injection(LVI)and transverse sinus injection(TSI)mediated adeno-associated virus expression in neonatal mice,and to provide reference for the selection of virus injection methods for the study of the function of specific brain regions in mouse development.Methods The newborn C57BL/6 mice were injected with rAAV-GAD67-EGFP-WPREs virus by LVI and TSI,respectively.Brain tissue samples were collected on the 24th day,and frozen brain slices were prepared to observe the expression range of virus-carried fluorescent protein in the mouse brain.Results The LVI method was easy to operate,but its range of virus injection was limited in the brain,mainly in the cerebral cortex.The TSI method was more complicated and required larger amount of virus,but more brain regions were infected under this method.Conclusion Different injection methods mediate the expression of virus in different brain regions,among which LVI is more suitable for the study of cerebral cortex development,and TSI is suitable for the developmental study of almost all brain regions.
lateral ventricletransverse sinusadeno-associated virusbrain development
维普
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