Studies on PC-3 cell apoptosis-inducing effect of C21 steroidal glycosides from Radix Cynanchi Auriculati by high performance centrifugal partition chromatography
Objective To extract and purify of C21 steroidal glycosides from Radix Cynanchi Auriculati and to study their apoptosis-inducing effects on PC-3 tumor cells. Methods By using high performance centrifugal partition chromatography, isolate and purify of C21 steroidal glycosides from the crude steroids of Radix Cynanchi Auriculati.The extract was tested by FCM and acridine orange (AO) staining fluorescence to study the apoptosis-inducing effect. Results FCM assay showed that the apoptosis-inducing effect was dose-depend with the extract, and the proportions of G2 cells and M cells increased; the results of AO staining fluorescence indicated that the extract induced nuclear fragmentation, pycnosis, formation of apoptotic body. Conclusion By using HPCPC, isolate and purify of C21 steroidal glycosides from Radix Cynanchi Auriculati, and the extract could induce apoptosis on PC-3 tumor cell and the arrest of cell cycle G2/M phase, which may be the one of mechanisms of anti-tumor effect against PC-3 cells.
NETL
NSTL
万方数据
维普
