Objective To confirm the preparation technology of Hu-Huang Burn Gel and establish its quality control method.Methods The formability and stability of the gels were taken as examining index by the single factor experiment,and through which the most appropriate matrix for the Chinese medicine extract was selected.The HPLC method was used for simultaneous determination of the content of polydatin and berberine hydrochloride in the formulation.Results 2.5％ HPMC-K4M was proved to be the optimum matrix.The linear regression equation of polydatin about concentration and peak area was Y=79 415X-444 889 (r=0.9994),the linear range was 15.6-78.0 μg/mL; the linear regression equation of berberine hydrochloride about concentration and peak area was Y=65 576X-583 154 (r=0.9996),the linear range was 34.75-173.75 μg/mL; and the respectively average recovery was 99.34％ and 99.64％ (n=6).Conclusion The preparation of Hu-Huang Burn Gel is simple and the nature of this formulation is stable,the assaying method is accurate with well reproducibility,which can promote the application of the formulation in the clinic.
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