Objective To screen the antitumor active fraction from Artemisia selengensis Turcz. by antitumor experi-mental method in vitro and in vivo. Methods Artemisia selengensis Turcz. were extracted with 95% ethanol eluent, then petroleum ether, chloroform, ethyl acetate, n-butyl alcohol extracted from the active fraction. The active fraction of good inhibitory effect on HepG2 cells proliferation was screened out by MTT assay. The inhibition effect of solid tumor by tumor-bearing mouse model was investigated. Results Ethyl acetate extract of Artemisia selengensis Turcz. could significantly inhibit the growth for cell proliferation, the inhibition rate was 15.64% at 10 g/L. The inhibition rate was 78.21% at 1000 g/L. With the increase of the concentration, inhibiting action increased. Ethyl acetate extract of Artemisia selengensis Turcz. significant inhibition effect of solid tumor. When the concentration of 400 mg/kg for tumor bearing mouse inhibition rate was 71.63%. Conclusion Ethyl acetate extract of Artemisia selengensis Turcz. has signif-icant anti-tumor activity in v itro and in v iv o.
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