Objective To optimize extraction technical parameters of Guci Kangfu pills,and to provide the evidence for industrial production.Methods The rate of extractum and sinomenine contents were selected as evaluation index;Sinomenine content was determined with the mobile phase of acetonitrile-ethlenediamine (32:68),the flow rate of 1.0 mL/min,detection wavelength 262nm,and HPLC welchrom C18 (250 mm×4.6 mm,5 μm) with column temperature of 30℃.The water absorption rate and the number of extraction times were investigated by the single factor experiment.The effects on the experimental results with different levels of the soaking time,the amount of water and the time of extraction was studied by orthogonal method.Results The Guci Kangfu pills optimum water extraction parameters:medicinal water absorption was 180.9％,extracting 2 times,the first with 10 times amount of water,extracting times was 2.5 h,second time with 8 times the amount of water,extracting time was 2 h.The optimum parameters of testing results showed that the extraction rate can reach 33.08％,RSD 0.64％ and the content of sinomenine can reach 16.7 mg/mL,RSD 1.99％.Conclusion The optimal water extraction process parameters are scientific,reasonable,stable and feasible,and suitable for the industrial production.
Guci Kangfu pillThe rate of extractumSinomenine contentsOrthogonal design testExtraction process
NETL
NSTL
维普
万方数据
