Objective To investigate the protective effect of fructus broussonetiae on L02 cell damage induced by acetaminophen(APAP).Methods The survival rate of normal L02 cells was determined by CCK-8 method at different concentrations(0.1,0.3,1.0,3.0,10.0 mg/ml).After the L02 cells were infected with APAP,the L02 cells with logarithmic growth stage were selected and divided into normal group,APAP group(40 mmol/L)and fructus broussonetiae group(1 mg/ml).The cell survival rate was determined by CCK-8 method,and the intracellular ROS levels were determined by DCFA-DH method.Apoptosis was observed by Hoechst 33258 staining.The expression of mitochondrial membrane potential in L02 cells was detected by Rhodamine 123 staining.The expression levels of JNK,p-JNK,78GRP78,and CHOP were detected by Western blot.Results Compared with 0.1 mg/ml fructus broussonetia water extract,the cell activity of 0.3,1.0,3.0,and 10.0 mg/ml fructus broussonetia water extract decreased,and the difference was highly statistically significant(P＜0.01).Compared with normal group,the cell survival rate and the average fluorescence intensity of apoptosis of APAP group were decreased(P＜0.05).Compared with APAP group,the survival rate of 1 mg/ml fructus broussonetia water extract increased,and the average fluorescence intensity of apoptosis decreased(P＜0.05).The ROS fluorescence inten-sity of APAP group was higher than that of normal group,and the differences were highly st atistically significant(P＜0.01).The ROS fluorescence intensity of fructus broussonetiae seed group was lower than that of APAP group,and the differences were highly statistically significant(P＜0.01).The average fluorescence intensity of mitochondrial membrane potential in APAP group was lower than that in normal group,and the difference was highly statistically significant(P＜0.01).The average fluorescence intensity of mitochondrial membrane potential in fructus broussonetia group was higher than that in APAP group,and the difference was highly statistically significant(P＜0.01).There was no significant difference in JNK protein expression among all groups(P＞0.05).Compared with normal group,the expression levels of P-JNK,GRP78,and CHOP protein in APAP group were increased(P＜0.01).Compared with APAP group,the protein expression levels of P-JNK,GRP78 and CHOP in fructus broussonetiae group were decreased(P＜0.01).Conclusion Fructus broussonetiae mitigates liver injury by inhibiting JNK,restoring mitochondrial membrane permeability,suppressing endoplasmic reticulum stress,lowering ROS levels,and inhibiting apoptosis.
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