Study on quality control of Chushi Wan by high performance liquid chromatography fingerprint combined with chemical pattern recognition
Objective To establish the fingerprint of Chushi Wan and to provide the reference for improving the quality control.Methods Luna Omega Polar C18 column(150 mm×4.6 mm,5 μm)was used in this high performance liquid chromatography(HPLC),mobile phase:acetonitrile-0.2%phosphoric acid aqueous solution,flow rate was 1.0 ml/min,wavelength was 254 nm,column temperature was 30℃,injection volume was 10 μl.The HPLC chromatograms of 29 batches of Chushi Wan were determinated and evaluated by the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint(2012 edition)with the reference of baicalein.Results There were 35 common peaks in the HPLC chromatograms of 29 batches of samples,the similarity was above 0.95 and eight chromatographic peaks were identified.Cluster analysis grouped 29 batches of Chushi Wan into three groups.The seven main components in principal component analysis reflected 32 common peaks information.Conclusion There are some quality differences in different batches of Chushi Wan.The established fingerprint can be used to evaluate the quality of Chushi Wan.
万方数据
维普
