Study on the influence of sample hemolysis on the detection of blood bio-chemical items in patients with paroxysmal nocturnal hemoglobinuria laboratory processing path
Objective To explore the study on the influence of sample hemolysis on the detection of blood biochemical items in patients with paroxysmal nocturnal hemoglobinuria laboratory processing path.Methods A total of 275 health check-ups were selected from the Physical Examination Center of the First Affiliated Hospital of Nanjing Medical University from November 2022 to April 2023.Simulated blood lysate was prepared by using 25 blood samples.Serum hemolysis correction equations were established by screening the blood biochemical items with large hemolysis serum detection bias(BH)accord-ing to the hemolysis index.Plasma and serum samples were collected from 250 cases,and the detection bias(BS)of each specified biochemical item was calculated among different specimen types,and then compared with the specified item anal-ysis bias(BA)to establish the plasma reference interval respectively.Results The results of 11 blood biochemical items were easily affected by hemolysis.The serum hemolysis correction equations of aspartate aminotransferase(AST),glutamyl-transpeptidase,lactate dehydrogenase(LDH),creatine kinase,α-hydroxybutyrate dehydrogenase,total protein(TP),creati-nine,and potassium ion(K+)were established using the hemolysis index as the horizontal coordinate and the mean value of BH as the vertical coordinate,and the R2 values were all greater than 0.90.As the Bs of AST,LDH,TP,phosphorus'and K+were all higher than the appropriate BA,the plasma reference intervals were established,which were(21.9±10.5)U/L,(166±58)U/L,(71.3±10.8)g/L,(1.14±0.37)mmol/L and(3.66±0.61)mmol/L,respectively.Conclusion In a certain degree of hemolysis,the serum hemolysis correction equation can be used to correct the results of some blood biochemical items.It is recommended to preferentially use patient plasma for testing,and then refer to plasma reference intervals for each item for more accurate reporting results.