Effect of olfactory training on olfactory function in mice with 3-methylin-dole-induced olfactory disorder
Objective To investigate the effect of olfactory training on the olfactory function of 3-methylindole induced olfactory dysfunction mice.Methods Sixty SPF grade male C57BL/6J mice aged eight weeks and weighing 18-22 g were selected and divided into control group,olfactory disorder group,and olfactory training group according to random number table method,with 20 mice in each group.The olfactory disorder model was established by 3-methylindole induction in the olfactory disorder group and the olfactory training group,and the model was successfully established with no rat granule found within 200 s.The olfactory training group received olfactory training,and inhaled four kinds of olfactory elements(rose essential oil,lemon essential oil,clove essential oil,and eolineol),each of which was continuously inhaled for 30 min,twice a day;meanwhile,the control group and the olfactory disorder group were given the same amount of distilled water atomization treatment,and the three groups were continuously interfered with for four weeks.Foraging time and olfactory epithelial function were compared among the three groups.Results The foraging time after setting up the model and after 1,2,4 weeks of training in olfactory disorder group was longer than that before modeling and in the same period of control group,the differences were statistically significant(P<0.05).The foraging time of olfactory training group after setting up the model and after 1,2,4 weeks of training was longer than that before modeling;the foraging time after 1,2,4 weeks of training was shorter than that after modeling,the foraging time after 2,4 weeks of training was shorter than that after training one week,and the foraging time after training four weeks was shorter than that after training two weeks,and the foraging time after 1,2,4 weeks of training was shorter than that in the same period of olfactory impairment group,the differences were statistically significant(P<0.05).After four weeks of training,the number of olfactory marker protein positive cells,the total number of olfactory epithelial cells,and the thickness of olfactory epithelial cells in the olfactory disorder group were lower than those in the control group,and those of olfactory training group were higher than those of olfactory disorder group,and the differences were statistically significant(P<0.05).Conclusion Olfactory training can shorten the foraging time of mice with olfactory impairment,improve the function of olfactory epithelium,and promote the recovery of olfactory function.
Olfactory disordersMice3-MethylindoleOlfactory trainingOlfactory function