Experimental study of sodium valproate in rats with brain injury at early stage of subarachnoid hemorrhage
Objective To investigate the effect of sodium valproate in rats with brain injury at early stage of subarachnoid hemorrhage.Methods A total of 120 healthy adult male SD rats were divided into sham operation group,model group,and sodium valproate group according to the random number method,with 40 rats in each group.Rats in sodium valproate group were given intraperitoneal injection of sodium valproate(300 mg/kg)every 12 hours.The apoptosis of cortical cells and neurons after subarachnoid hemorrhage was detected by TUNEL staining.Results The basal cerebral artery was clearly visible in the sham operation group,and the subarachnoid hemorrhage was obvious in the model group.Intra-group comparison:compared with the first day,the modified Garcia score of rats in model group and sodium valproate group at 3 and 4 days was increased,the differences were statistically significant(P＜0.05).Comparison between groups:in the first 3 days,the modified Garcia score of rats in the model group was lower than that in the sham operation group,(P＜0.05);on day 4,the modified Garcia score of rats in sodium valproate group was higher than that in model group,and the difference was statistically significant(P＜0.05).The proportion of positive cells in the cortex of rats in the model group was higher than that in the sham operation group,the difference was highly statistically significant(P＜0.01);while the proportion of positive cells in the cortex of rats in the sodium valproate group was lower than that in the model group,the difference was statistically significant(P＜0.05).Conclusion Sodium valproate can reduce the apoptosis of cells and neurons,and improve nerve injury and dysfunction after subarachnoid hemorrhage.
万方数据
维普
