Changing characteristics of chemical composition of different leaves of Aconitum carmichaelii Debx.and the effect of topping on root
Objective To clarify the distribution of chemical components in leaves of Aconitum carmichaelii Debx.and to provide scientific basis for topping technology based on the production technology of topping in traditional cultivation of Aconitum carmichaeli Debx.,the chemical components in different positions leaves of Aconitum carmichaelii Debx.were syst ematically analyzed,and the yield and composition of roots before and after topping were compared.Methods UPLC-Q-ToF-MS technology was used,with acetonitrile-0.1%formic acid water as the mobile phase,the column temperature was 45℃,and the flow rate was 0.4 ml/min,diterpenoid alkaloids in apex leaves,diverse leaf sections(first six leaves),and both pre-and post-pruned root samples of Aconitum carmichaelii Debx.was employed detected,and multivariate statistics evaluated topping process effects.Results The apex leaves,disproportionately enriched in diterpenoid alkaloids,had 60 chemical compounds detected,including 25 distinct ones.The specific components were monoester,diester,and trester diterpenoid alkaloids.Compared with the apex leaves,the number of diterpenoid alkaloids in comparatively developed leaves(1-6 leaves)dramatically drops,with a decrease trend of trester ≈ diester＞monoester＞ethanolamine,that is,the content of diester and trester diterpenoid alkaloids decreased rapidly in mature leaves.After topping process of Aconitum carmichaelii Debx.,the yield of roots increased by 10%per aconite.Analysis validated significant differentiation in underground root components post-pruning,such as Songorine,7-deoxycotonine,Fuziline,chasmanine,14-acetyltalatizamine,10-OH mesaconitine,and mesaconitine.Conclusion The apex leaves of Aconitum carmichaelii Debx.are rich in distinctive diterpenoid alkaloids,and pruning prominently affects their distribution and significantly boosts yield.
Aconitum carmichaelii Debx.Apex leafDiterpene alkaloidsTopping process
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