Effect of icariin on proliferation and osteogenic differentiation of bone marrow stromal stem cells in fractured rats
Objective To explore the effect and possible mechanism of icariin on proliferation and osteogenic differentiation of bone marrow stromal cells(BMSCs)in fractured rats.Methods Five SPF-grade SD rats were selected,eight weeks old,male or female,weighing(290±20)g.The rats were anesthetized,and the intercondylar cavity of the femur was exposed.After drilling the condyle,the Kirschner needle was inserted into the tibial pulp cavity,and the femur was amputated with a hacksaw to form a transverse fracture and sutured.Two days after the fracture,the neck was severed after anesthesia,the femur was stripped,and BMSCs were isolated in vitro.The 3rd generation BMSCs were divided into control group(no special treatment),low-,medium-,and high-dose groups(adding icariin solution with final concentration of 1,10,and 100 μmol/L).The proliferation,alkaline phosphatase(ALP)activity,mineralized nodule area,Janus kinase 2(JAK2),signal transducer and activator of transcription 5(STAT5),and p-STAT5 expression of BMSCs in each group were detected.Results Compared with control group,the optical density(OD)value,ALP activity,and the proportion of mineralized nodule area of low-,medium-,and high-dose groups were increased,JAK2 and p-STAT5/STAT5 were decreased(P＜0.05);compared with low-dose group,OD value,ALP activity,and the proportion of mineralized nodule area of medium-and high-dose groups were increased,JAK2 and p-STAT5/STAT5 were decreased(P＜0.05);compared with medium-dose group,OD value,ALP activity,and the proportion of mineralized nodule area of high-dose group were increased,JAK2 and p-STAT5/STAT5 were decreased(P＜0.05).Conclusion Icariin may promote the proliferation and osteogenic differentiation of BMSCs in rats with fracture trauma by inhibiting JAK2/STAT5 pathway.
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