Discussion on the potential targets and mechanisms of postmenopausal osteoporosis based on differentially expressed genes
Objective To obtain the differentially expressed genes of postmenopausal osteoporosis based on Gene Expression Omnibus(GEO)microarray data,and to explore their potential targets and mechanisms.Methods The microar-ray data of postmenopausal osteoporosis were screened from GEO database,processed by GEO2R software to obtain differ-ential genes,and standardized and verified by UniProt database.The proteinprotein interaction(PPI)network was obtained by using STRING database.The PPI network was constructed by using Cytoscape 3.9.1 and topological analysis was per-formed to screen core genes.The R packages such as clusterProfiler and pathview were used to analyze the gene ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway involved in differential genes,and Weishengxin Platform was used to visualize the results.Results A total of 656 differential genes were obtained,including 421 down-regulated genes and 235 up-regulated genes.Ten core genes were obtained,namely VEGFA,KRAS,EP300,IL-2,IL-4,KIT,FOS,CCND1,HGF,and CYCS.The results of molecular function,biological process,and cell component enrichment in GO were 771,4 648,and 499,respectively.The molecular functions were mainly concentrated in lipid binding,phospholipid binding,and so on,the biological processes were mainly concentrated in the regulation of peptidase activity,the negative regulation of proteolysis,and so on,and the cellular components were mainly concentrated in the endocytic vesi-cles,the external side of the plasma membrane,and so on.There were 299 KEGG pathway enrichment results,mainly involving PI3K-Akt signaling pathway,viral protein interaction with cytokines and cytokine receptors,B cell receptor signaling pathway,hematopoietic cell lineage,Ras signaling pathway,etc.Conclusion The selected differential genes and related signaling pathways are helpful to further understand the target and mechanism of postmenopausal osteoporosis,and provide new ideas for the development of new drugs.
Postmenopausal osteoporosisGene Expression OmnibusTarget and mechanism
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