Study on the effect of Juzao Pills drug-containing serum in regulating PTEN on the growth and metastasis breast cancer MCF-7 cells
Objective To study the effects of Juzao Pills drug-containing serum in regulating the expression of phosphatase and tensin homolog(PTEN)gene on the growth and metastasis of human breast cancer MCF-7 cells.Methods A total of 12 SPF female Wistar rats eight weeks old weighing(200±20)g were divided into Juzao Pills group and control group by random number table method,with six rats in each group.Each group was administered with 5.4 g/(kg·d)Juzao Pills or equal volume of saline by gavage to prepare drug-containing serum and blank control serum.The cultivated human breast cancer MCF-7 cells were divided into 20%blank control serum group,5%drug-containing serum group,10%drug-containing serum group,15%drug-containing serum group,and 20%drug-containing serum group,which were intervened with culture medium containing 20%blank control serum,5%,10%,15%,and 20%drug-containing serum,respectively.Then CCK-8 was used to detect cell proliferation,colony formation experiment was used to observe clonogenic ability,TUNEL staining was used to detect apoptosis,flow cytometry was used to detect cell cycle distribution,scratch test,and Transwell was used to detect cell migration and invasion abilities,qPCR and Western blot were used to detect PTEN mRNA and protein expression levels.Results Compared with 20%blank serum group,the cell proliferation activity,the cloning efficiency and the invasion ability were decreased in 10%,15%,and 20%drug-containing serum groups(P＜0.05).In 5%,10%,15%,and 20%drug-containing serum groups,apoptosis level was increased,migration ability was decreased,cell cycle was blocked in G 1 phase,mRNA and protein expression level of PTEN were increased(P＜0.05).Compared with 5%drug-containing serum group,the cells in 10%,15%,and 20%drug-containing serum groups proliferation activity was decreased,cloning efficiency decreased,apoptosis level was increased,migration ability was decreased,invasion ability was decreased,cell cycle was blocked in G 1 phase,and PTEN mRNA and protein expressionlevels increased(P＜0.05).Conclusion Juzao Pills drug-containing serum could inhibit the growth and metastasis of MCF-7 cells,and its mechanism may be related to the activation of PTEN expression.
Breast cancer cellsJuzao PillsPhosphatase and tensin homologyDrug-containing serumMCF-7 cells
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