Effect of Hexokinase domain component 1 gene knockout on the prolifer-ation,migration,and invasion ability of human colorectal cancer cells
Objective To investigate the effect of hexokinase domain component 1(HKDC1)on the proliferation,migration,and invasion of human colorectal cancer cells.Methods HT-29 and SW480 cells were transfected immediately,and HKDC1-siRNA group,negative control group,and blank group were set up.Meanwhile,colorectal cancer tissues and adjacent normal tissues of 50 patients who underwent surgery in the First Affiliated Hospital of Hebei North University from March 2020 to May 2022 were collected.The expression of HKDC1 mRNA was detected by RT-qPCR;the expression of HKDC1 protein was detected by Western blot;cell proliferation,migration,and invasion were detected by CCK-8 assay,scratch assay,and Transwell assay.Results The expression level of HKDC1 mRNA in HT-29 cell was higher than that in SW480 cell(P＜0.05),therefore,HT-29 cell lines were selected for subsequent experiments.The expression levels of HKDC1 mRNA and optical density value in colorectal cancer tissues were higher than those in adjacent normal tissues(P＜0.05).There were no significant differences in the expression levels of HKDC1 mRNA and protein between blank group and negative control group(P＞0.05);the expression levels of HKDC1 mRNA and protein in HKDC1-siRNA group were lower than those in negative control group(P＜0.05).There was no significant difference in optical density value between blank group and negative control group(P＞0.05);the optical density value of HKDC1-siRNA group was lower than that of negative control group(P＜0.05).There was no significant difference in cell migration rate between blank group and negative control group(P＞0.05);the cell migration rate of HKDC1-siRNA group was lower than that of negative control group(P＜0.05).There was no significant difference in the number of cell migration between blank group and negative control group(P＞0.05);the number of cell migration in HKDC1-siRNA group was lower than that in negative control group(P＜0.05).Conclusion HKDC1 is highly expressed in colorectal cancer tissues and cells,which can promote the proliferation,migration,and invasion ability of colorectal cancer cells,and is expected to become a new target for colorectal cancer gene therapy.
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