Effect of rhein on lipopolysaccharide induced polarization phenotype of microglia and related inflammatory factors
Objective To investigate the effect of rhein on lipopolysaccharide(LPS)induced polarization phenotype of microglia and related inflammatory factors.Methods Microglia were cultured in vitro and divided into blank group,LPS group,low,medium,and high dose rhein group,blank group was cultured routinely,and the remaining four groups were induced by the addition of 1 μg/ml LPS intervention,normal medium was given to the LPS group,and 1,10,and 20 μmol/L of rhein was given to the low,medium,and high dose rhein group,respectively.Microglia morphology was observed by inverted microscope;microglia activity was detected by CCK-8;the ratio of CD86+(M1-type)and CD206+(M2-type)in microglia was detected by flow cytometry;and the content of interleukin(IL)-1 β,IL-6,and tumor necrosis factor-α(TNF-α)in the cellular supernatant was detected by enzyme linked immunosorbent assay,and inducible nitric oxide synthase(iNOS)activity was determined by kit.Results Rhein at different concentrations had no significant effect on the morphology and proliferation of LPS-induced microglia.Compared with blank group,CD86+expression was increased and CD206+expression was decreased in LPS group(P＜0.05).Compared with LPS group,the expression of CD86+in medium and high dose rhein groups was decreased,and the expression of CD206+was increased(P＜0.05).Compared with low dose rhein group,CD86+expression decreased and CD206+expression increased in medium and high dose rhein groups(P＜0.05).Compared with blank group,the expression of IL-1 β,IL-6,and TNF-αin LPS group were increased(P＜0.05).Compared with LPS group,the expressions of IL-1,IL-6,and TNF-α in medium and high dose rhein groups were decreased(P＜0.05).Compared with low dose rhein group,the expressions of IL-1,IL-6,and TNF-α in medium and high dose rhein groups were decreased(P＜0.05).Compared with blank group,iNOS expression in LPS group was increased(P＜0.05).Compared with LPS group,iNOS expression in low,medium,and high dose rhein groups was decreased(P＜0.05).Conclusion Rhein converts the LPS-induced microglia polarization phenotype from M1-type to M2-type,which subsequently reduces the release of relevant inflammatory factors to exert its anti-inflammatory effects.
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