Thin-layer chromatography identification and content determination of Baihua Ningsou Lu
Objective To explore the identification of ephedra,platycodon platycodon,and bitter almond and the content determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in ephedra in hospital preparation Baihua Ningsou Lu,and to provide the basis for the quality control of Baihua Ningsou Lu.Methods According to the fourth part of Pharmacopoeia of the People's Republic of China(general rule 0502),the ephedra,platycodon grandiflorum,and bitter almond in Baihua Ningshou Lu were identified by thin-layer chromatography,and the con-tents of ephedrine hydrochloride and pseudoephedrine hydrochloride in ephedra were determined by high performance liquid chromatography.The chromatography column was performed on YMC Hydrosphere C18(4.5 mm × 250 mm,5 μm),acetonitrile∶0.2％ phosphoric acid solution(4∶96)as the eluent,isocratic elution,and the elution time was 20 min.The detection wavelength was 210 nm,the flow rate was 1.0 ml/min,the column tem-perature was 35 ℃,and the sample size was 5 μl.Results Ephedra,platycodon grandiflorum,and bitter almond developed well,with clear spots and obvious experimental bands.Ephedrine hydrochloride and pseudoephedrine hydrochloride had good linear relationships in the ranges of 1.369 3-27.386 7 μg/ml and 1.058 7-21.173 3 μg/ml,respectively;and the regression equations were Y=0.153 4X+0.009 8(r=0.998 9),Y=0.140 8X+0.004 7(r=0.999 1);the average recoveries were 102.6％and 101.5％,and the RSD values were 3.7％ and 1.5％,respectively.Conclusion The method can identify Baihua Ningsou Lu qualitatively and determine the content of effective components.It is simple,reproducible and stable,and can be used for the quality control of Baihua Ningsou Lu.
Baihua Ningsou LuThin-layer chromatographyIdentificationContent determinationQuality control
万方数据
维普
