Effects of human immunodeficiency virus infection during pregnancy on vaginal microbiota
Objective To investigate the characteristics of vaginal microbiota in pregnant women infected with human immunodeficiency virus(HIV).Methods Pregnant women who underwent prenatal examination in Beijing Youan Hospital from October 2021 to May 2023 were selected as the study objects,among which 23 pregnant women with confirmed HIV infection were taken as the HIV group and 23 normal pregnant women were taken as the control group.General information about the subjects were collect;vaginal secretions of the subjects were collected,vaginal bacterial genomic DNA was extracted,and gene sequencing and microbial community analysis were performed on the V3-V4 highly variable region of bacte-rial 16S rRNA gene;the effective sequences were divided into different operational taxonomic units(OTU)with 97％similarity,and the sequences with the highest abundance were selected as the OTU representative sequences.The species composition,Alpha diversity,and Beta diversity of the two groups were compared to find the different species between groups.Results In this study,a total of 31 phyla,78 classes,202 orders,385 fami-lies,796 genera,and 1 024 species level species were annotated.At the species level,the dominant bacteria in the HIV group was Lactobacillus in-ert,and the dominant bacteria in the control group was Lactobacillus crispatus.Compared with the control group,the proportion of Lactobacillus crispatus decreased in HIV group,and the proportion of Lactobacillus inert,Lactobacillus Jenni and other anaerobic bacteria increased.Alpha di-versity analysis showed that there were no statistical significances in ACE index,Chao1 index,Simpson index,and Shannon index between the two groups(P＞0.05).Beta diversity analysis showed that the species composition of vaginal flora was similar between the two groups.Conclusion The imbalance of vaginal flora in pregnant women with HIV infection during pregnancy showes that the proportion of Lactobacillus crispatus decreased,and the proportion of Lactobacillus inert and other anaerobic bacteria increased.
Human immunodeficiency virusPregnancyVaginal microbiotaSpecies composition
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